Our ongoing series Life of a Sample explores what happens behind the scenes at WETLAB.  If you missed part one, two, or three, check them out here!

The next step for our samples life cycle is analysis, which takes around four days, depending on the method.  Analysis starts with batch prepping the samples and preparing the instrument.  All daily required instrument maintenance is also preformed during this step.  All reagents and standards are logged here, and will later be used to monitor for QA/QC and determine accuracy.  When the batches and machines are prepped, the samples are run through analysis.  During this time, all quality control samples are monitored to ensure that the machines are functioning properly and there are no problems with analysis.  If any issues or unexpected results appear, analysts will trouble shoot all instruments and samples.  Samples that do not meet acceptance criteria are then re-run.  Potential problems can arise when samples have complicated matrices, which can cause equipment malfunctions. Once our samples are done in the analysis stage, it’s on to data entry.

A few of WETLAB’s state of the art analysis instruments.

Have you ever wondered what happens behind the scenes at WETLAB?  You drop off a sample, then ten days later, you receive results.  Simple, right?  Well, there is a lot that happens from the time a cooler full of samples comes in the door to the time that you get results.  In this six-part series, Life of a Sample, you will get an inside look at what happens as a sample flows through the lab.  At WETLAB, we pride ourselves on the quality of our work, and the way that we handle your project exemplifies that. 

The first part of sample life begins before a specimen has ever been collected.  We begin with pre-project planning, which involves determining how WETLAB can make your project a reality.  Our stellar project managers work with you to figure out what you need done, and how we can make it happen.  This involves liaising with our lab manager, quality assurance manager, operations manager, and business development manager to ensure that we have the proper resources in place to preform all required and requested testing services.  Once a project scope has been discussed and agreed to, you gather your samples and bring them to lab (or have one of our friendly couriers pick them up).  When the samples arrive in the lab, the real fun starts.

A sample is received, along with a detailed Chain of Custody, by one of our knowledgeable client service representatives.  The sample is then logged in and checked for any initial errors in preservation, temperature, etc.  After that, the samples undergo two levels of quality assurance and control before they ever reach the lab.  The first is primary entry into the laboratory management system.  Once the primary entry is done, the sample is split into the necessary sets, and preserved and/or filtered if needed.  When the samples are in the proper containers for analysis, a second set of eyes reviews them and the initial data entry to ensure accuracy.  After the secondary review, the samples are ready to be released to the lab to begin analysis.  This intensive initial receiving takes about four hours, ensuring plenty of time for the next five steps to occur.  Next up: Step 2, Sample Preparation.

Client Services staff doing primary and secondary review of new samples.

Project Manager Hollie checking a sample against a COC to ensure initial accuracy.

The Method Detection Limit (MDL) is defined as “the minimum concentration of a substance that can be measured and reported with a 99% confidence that the analyte concentration is greater than zero” (40CFR sec. 136 Appendix B). The MDL is used for various reasons in an analytical laboratory, with the primary reason being it is used to determine the reporting limits for each analyte that a laboratory analyzes for. Reporting Limits (RL’s) are generally 3-5 times the MDL. On occasion a laboratory can report lower than the calculated MDL, however these results are always flagged as estimates and cannot not always be considered legally defensible data. MDL studies are also required as part of the process of maintaining certifications.

A MDL is initially established when a method is set up, a new piece of equipment is brought online or if there is a significant change in equipment or location (i.e. moving to a new location, significant maintenance or replacement of major parts). MDL’s are confirmed on a regular basis according to method specifications (generally on an annual basis but for some parameters, such as anions, it is done twice a year). MDL studies are performed on a regular basis to assure that there is no loss of sensitivity on the equipment due to wear and tear or the constantly changing environmental conditions.

Do you have more questions about MDL’s or questions about other laboratory terminology? Is there something that always confused you about laboratory services? Leave a comment and let us know what you want to know about!

WETLAB’s objective is always to produce the highest quality data while providing our clients with superior customer service. Our client services staff is renowned for both meeting the testing needs of our clients’ and offering innovative and customized solutions. In response to the needs of many of our regional clients, we have developed niche products and services specific to Northern Nevada and Northern California.

WETLAB routinely provides comprehensive analytical support on a variety of matrices including:

• Wastewater
• Surface water
• Storm water
• Soil
• Wastes
• Rocks
• Groundwater
• Drinking water
• Sludge
• Filters
• Monitor wells
• Speciality Matrices (De-icing products, media, paint chips, etc.)

Compounds for which WETLAB provides services include:

• Metals
• Anions
• Low Level Nutrients
• MWMP Extractions
• Microbiology
• General Chemistry
• TCLP Compounds
• Priority Pollutants
• Mining Chemistry

If you would like a complete listing of parameters that we perform testing on, email us at ginger@wetlaboratory.com.

We perform testing to comply with the following programs in Nevada and California:

• NPDES
• RCRA
• SDWA
• CWA
• We also appear of the Bureau of Reclamation’s Approved Vendor List

Our quality assurance program ensures that data is produced in an accurate, precise, legally defensible, timely and cost effective manner. Our Quality Assurance Plan provides the structure, policies and responsibility for the execution of quality assurance, quality control and quality assessment programs. We have also developed Standard Operating Procedures for all of the methods and procedures that are performed in our laboratories. If you are interested in receiving a copy of our Statement of Qualifications or Quality Assurance Plan, please email Ginger Peppard at ginger@wetlaboratory.com.